Abstract

Regeneration of Biomimetic Hydroxyapatite on Acid Etched Human Enamel using PAMAM Template in Vitro

Introduction: Traditional dental restorative materials do not fit well with the natural tissue at the lesion interface because their components, structure, and properties are not similar to those of the natural hydroxyapatite. Objective: To mimic the natural biomineralization process regulated by amelogenin in developing enamel using a PAMAM template. This work will explain the nature of PAMAM-PO₃H₂ with acid-etched enamel in a mineralization solution. Methodology: Here, we attempted to use a phosphate-terminated PAMAM dendrimer and investigate its application as an amelogenin analog on the remineralization process of acid-etched enamel. The fourth generation PAMAM-PO₃H₂ was arranged. For the mineralization solution, a 3 ml calcification solution containing 2.58 mM CaCl₂ and 1.55 mM KH₂PO₄, 20 mM HEPES, and 180 mM NaCl was arranged. NaOH will be used to adjust the pH of the mineralization solution and fluoride will be obtained from NaF. Tooth samples with intact enamel were obtained. The roots were separated from the teeth and the enamel samples were cut longitudinally to obtain 3x3x1 mm samples. These samples were coated with PAMAM-P0₃H₂ and reacted with the mineralization solution. Conclusion: This will help us understand the biomineralization process in enamel formation. The application of PAMAM with phosphate and mineralization solution could result in the formation of biomimetic hydroxyapatite.

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