Background: Nano-biotechnology pertains to biologically driven frameworks and evolution for developing wares at the Nanoscale. We explored Nano-biotechnology in this article, which is a unique multidisciplinary line of research based on the joint endeavor of biologists, chemists, physicists, engineers, and medical specialists. Different principles used in Nano-biotechnology include the electron pair combination, electron pair bond polymerization, individual organization, and self-convocation. Objectives: The invention of Nanoparticles has revolutionized this field. Nano-particles are of various types such as carbon-based Nanoparticles, polymeric, ceramic, and metallic Nano-particles. They are synthesized through specific techniques which comprise bottom-up and top-down methods. These Nanoparticles are useful in various processes like in stalking of stem cells, gene delivery, and drug delivery as well as in environmental remediation. Methods: Nano-biotechnology has diversified applications in bio-formulations, Nanofabrication, biomimetics, and the production of fuel and energy. The development of Nano-robots is another marvelous achievement of Nano-biotechnology. Results: Nano-biotechnology has also conquered the field of medical science through its prominent hallmarks in cancer diagnostics, drug delivery, gene delivery, and stem cell-based therapy. Conclusions: This article also focuses on the latest advancements and the future prospectus of Nano-biotechnology. However, there are certain limitations and challenges in this field that could not be rejected but scientists are working to bring improvements and innovations to this burgeoning field
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