Abstract

In vivo Study of the Effect of Local Application of Melatonin and Beta-Tricalcium Phosphate on Healing of Experimentally Induced Bone Defect

Introduction: Bone healing is a highly complex regenerative process that the musculoskeletal system undergoes in order to restore skeletal integrity. Medical management for bone defects has been fundamentally absorbed in exchanging the lacking bone with bone grafts material. Aim of the study: The study aims to evaluate the effect of the combined local application of melatonin and ß-tricalcium phosphate (β-TCP) on the healing of induced bone defect by histological and histomorphometric analysis. Materials and methods: Total 24 adult male rats were divided into control and experimental groups, induction of femoral holes was done in both sides, whereas combined local application of melatonin and beta-tricalcium phosphate was done in one hole where the other hole was used as a control. The histological and histomorphometric analysis was done for all the specimens after scarification of animals for 1,2 and 4 weeks healing periods. Results: According to the findings of this study, the deposition of bone at operating sites was enhanced and accelerated after application of both melatonin and beta-tricalcium phosphate as mean values of bone trabecular area and number increased more than in control groups, and bone marrow area values decreased. Highest mean values of bone cells (osteoblasts and osteocytes) were detected in experimental groups. Osteoclast values decreased with time in the experimental group. In the control group showed the highest values in 2-weeks. Conclusion: Results of this study showed that the combined application of melatonin and betatricalcium phosphate was effective in the acceleration of the bone healing process

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