Abstract

IN VITRO ANTIOXIDANT ACTIVITY ASSESSMENT ON ALCOHOLIC EXTRACT OF CYANOBACTERIA CULTURES FROM SPIRULINA PLATENSIS

The antioxidant supplementation such as vitamin C, E, A and beta carotene etc, has proven to prevent the process of ageing caused by free radicals. Antioxidants either prevent free radical production or counteract the impeding damage by doing so they are involved in the protection and prevention of cellular damage which is the common pathway for cancer, aging and much disease. The cyanobacteria is a blue green micro algae believed to be a rich antioxidant source, found in abundance in Spirulina platensis is evaluated for antioxidant potential by modern in vitro techniques. METHODS the alcoholic extract of cyanobacteria is studied by in vitro Lipid Peroxidation inhibition assay (LPO) , The nitric oxide (NO)free radical scavenging activity and the determination of the DPPH (one, 1-diphenyl-2- picrylhydrazil) radical scavenging activity in concentrations which ranged from 1.95 g/mg to 500 g/mg in a geometric progression using spectrophotometer Results: The IC-50 % inhibition value of cyanobacteria by LPO is 102.70 mcg/ml, DPPH is 63.35 mcg/ml and by NO is 8.80 mcg/ml CONCLUSION The alcoholic extract of corynebacterium culture from Spirulina platensis exhibited potent NO free radical scavenging activity with mild activity on DPPH and LPO inhibition assay.

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