Professional behavior of nurses is basic concepts which depends on several individual and organizational factors. In recent decades much attention has been given to organizational culture and organizational commitment. Therefore, the aim of this study was to evaluate nurses' professional behavior and its relationship with organizational culture and commitment. This descriptive correlational study was done on 200 nurses from hospitals affiliated to Iran University of Medical Sciences in 2015, using stratified random sampling. To collect the data, demographic information, Goze professional behavior questionnaire, Robbins organizational culture questionnaire and Allen & Meyer organizational commitment questionnaire were used. Chi-square test and Pearson and Spearman correlation were used to analyze the data. The mean score of nurses' professional behavior was 108.98 ± 17.41, the mean score of organizational culture was 42.56 ± 17.32 and the mean score of organizational commitment of nurses was 97.94 ± 8.66. Pearson correlation coefficient analyses did not show significant correlation between professional behavior and organizational culture (P-value = 0.706 and R =0.027) and organizational commitment (P-value = 0.152 and R =0.102).The nurses' professional behavior did not have significant correlation with organizational commitment except normative commitment and did not have significant correlation with organizational culture except of attention to outcomes
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