Abstract

Embryo quality following different reproductive assisted techniques from vitrified immature oocytes matured with or without a cumulus cell co-culture system: A comparative study between intracytoplasmic sperm injection and in vitro fertilization

Author(s):Maryam Davari, Masih Allah Shakeri, Pejman Hamedi Asl, Mina Shariati, Mohamed Amin Ghobadifar, Hossein Kargar Jahromi, Navid Kalani and Farideh Mogharab

To consider which methods of micromanipulation techniques increases the human oocyte maturation in vitro and fertilization rate cultured with or without CCs, we aimed to compare intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF). Five hundred fifty immature oocytes were retrieved and were randomly divided into two groups; oocytes that were cultured with CCs (Group A) and oocytes cultured without CCs (Group B). After in vitro maturation (IVM), only oocytes that displayed Metaphase II (M II) stage went randomly through the ICSI or IVF procedure. Maturation and fertilization rates were all examined. The mean age, basal follicle-stimulating hormone (FSH), and number of oocytes recovered for the patients were all comparable between the two study groups. The number of oocytes that reached M II (mature oocytes) was 194 out of 275in group A(CC-co cultured group) compared to 165/275 in group B (P = 0.009). The fertilization rates of matured human oocytes cultured with and without CCs by ICSI procedure was significantly higher than IVF method (P = 0.005 vs. P = 0.001, respectively). Findings of the current study revealed that the fertilization rate of in vitro matured oocytes during ICSI procedure is higher than IVF method.

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