International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)
Vinod R. Ragade
Department of Zoology, V.G. Vaze College, Mulund, Mumbai, IndiaPublications
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Research
Induction of Apoptosis and Cell Cycle Arrest in Human Breast Cancer Cells T-47D and MDA-MB-453 Treated with Polyalthia longifolia Methanolic Extract
Author(s): Kiran Ramesh Kharat, Vinod R. Ragade, Preetha Achary and Amol Kharat*
Polyalthia longifolia cv. pendula (Family Annonaceae) contains active phytochemical moieties in various parts of the plant providing a valuable source for curative strategies in ailments and diseases. In the present study, the efficacy of Polyalthia longifolia leaves Methanolic Extract (PLME) was validated against two different human breast cancer cell lines-invasive ductal carcinoma cells-T-47D and adenocarcinoma cells-MDA-MB-453 cells. This reinforces the first report emphasizing the induction of apoptosis in PLME treated MDA-MB-453 cells. After 24 hours, the IC50 value of PLME was observed as 23.58 ± 3 μg/mL and 17.1 ± 1.2 μg/mL in T-47D and MDA-MB-453 cells, respectively. The early apoptotic population in PLME (20 μg/ml) treated T-47D cells was found to be 24.64% by the Annexin-V method. In MDA-MB-453 cells, 50.4% and 66.1% early apoptosis was recorded in 20.. Read More»
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