Research
Phenotypic Characterization of Acinetobacter spp. Isolated from Ventilator-Associated Pneumonia in an Intensive Care Unit of Tertiary
Care Hospital
Author(s): Vijeta Sharma*, Rajni Sharma and Aruna Vyas
Acinetobacter spp. has been recognized as one of the important causes of hospital-acquired infection in Ventilator- Associated Pneumonia (VAP). The emergence of Multidrug Resistance (MDR) Acinetobacter spp. has shown resistance to most available antibiotics especially in the intensive care unit. A total of 100 Acinetobacter spp were collected from 272 endotracheal aspirates among the VAP patients. The Kirby Bauer Disk Diffusion Method was used to conduct an antibiotic susceptibility test on quantitative cultures. The Phenotypic Confirmatory Disk Diffusion Test (PCDDT) was used to classify ESBL producers. Combined Disk Test detected MBL producers. Carbapenemase activity was detected by Modified Hodge Test (MHT) and these strains were subjected to E-Test for further confirmation of carbapenemase activity. Acinetobacter spp were found highly resistant to most of the cephalosporin but 100.. Read More»
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