International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)
Shreyas S Kuduvalli
Central Inter-Disciplinary Research Institute, Sri Balaji Vidyapeeth, Puducherry, IndiaPublications
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Research
Correlation of Sputum mir-144 Copy Levels with Treatment Response among Pulmonary Tuberculosis Patients
Author(s): Vimal Raj R*, Anitha TS, Pajanivel R, Shreyas S Kuduvalli and Adithan C
Background: MicroRNAs are approximately 22 nucleotides (nt) in length, small non-coding RNAs (ncRNAs), playing a vital role during post-transcriptional regulation of mycobacterial infection. Few sputum microRNAs (sputum miR) were evaluated in literature for their role as potential biomarkers in diagnosing Pulmonary Tuberculosis (PTB), but the correlation with a full course of Tuberculosis (TB) treatment is yet to be ascertained. Methods: Sputum samples of forty-six PTB patients were collected for Acid Fast Bacilli (AFB) staining and sputum microRNA-144 (sputum miR144) copy analysis, before, during, and at the end of treatment as per Indian National guidelines. Twelve patients of Asthma or Chronic Obstructive Pulmonary Disease (COPD), not in infective exacerbation, were recruited as controls. Sputum miR-144 copy levels were measured by quantitative Real-Time PCR (qRT-PCR) method. pResu.. Read More»
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