International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)
Eugene Ifeanyichukwu Ikeh
Department of Medical Microbiology, Faculty of Clinical Sciences, College of Health Sciences, University of Jos, NigeriaPublications
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Research
Paradigm Shift in Malaria Parasite Density Determination to First Principle Protocol
Author(s): Iseimokumo Christopher Peletiri*, Blessing Ukamaka Osita, Nkechi Adaoma Nwoke, Joseph Salifu David and Eugene Ifeanyichukwu Ikeh
Background: The need for a simple and more accurate malaria diagnosis and parasite density determination cannot be over-emphasized at such a time when there is increased infection prevalence and higher mortality rate as reported by the World Health Organization. Objective: This article highlights the simple novel approach in using the ‘first principle’ protocol on malaria parasite density determination. Methods: The protocol based on the first principle on malaria parasite quantification was used. The diameter of the field of view (dFOV, 0.178 mm) multiplied by 100 fields gives the diameter of the circle of thick blood film required. The volume of blood in the thick film is equal to the diameter of the circle of thick blood film divided by the volume of blood used (8 μL) and further divided by the m.. Read More»
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