International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)
Ammar ME. Hassan
University of Gezira, Wad Medani, SudanPublications
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Research
Status of H2AX and TP53 (Non- phosphorylation) for DNA Damage in Cancer Patients on Radiotherapy-A Case-Control Study
Author(s): Alkhansa S. Mahmoud*, Omer M. Abdalla, Amna A. Ali, Nuha M. Hassan, Amna A. Yousif, Fawzia E. Elbashir, Ahmed Omer, Raheel Mussa and Ammar ME. Hassan
Introduction: Radiotherapy is widely used in the treatment of cancer patients, and some genes affect the response to radiotherapy. In addition, phosphorylation is a hallmark of DNA damage response. In this study, we aim to investigate the status of H2AX and the non-phosphorylation forms of tumor suppressor p53 (TP53) before and after radiotherapy to reveal their roles in DNA damage response and radio-sensitivity. Materials and Methods: ELISA technique was used to determine the H2AX concentration and TP53 expression status in plasma of 29 cancer patients and 29 healthy individuals of the same age and sex as a control group. Patients were treated with radiotherapy, while the control group was not irradiated. H2AX and TP53 were measured before the start of radiotherapy on the first day of treatment and then 30 minutes after radiotherapy on the last day. Results: Both H2AX and TP53 had hi.. Read More»
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