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The Prevalence of TEM-1 gene causing resistance to beta-lactam antibiotics in Klebsiella pneumoniae isolates from clinical samples and plasmid curing

Authors: Ghanadian, Abdolhamid and Shabani Ali Akbar

Int J Med Res Health Sci.557-561 | pdf PDF Full Text

Existence of extended spectrum B-lactamase ( ESBL) genes plays an important role in causing B-lactam antibiotic
resistance in the producing strains of these enzymes. The resistance of gram-negative bacteria, such as klebsiella
pneumonia, to different antimicrobial agents, especially B-lactams and carbapenem, has increasingly been
reported. This study was conducted to determine the prevalence of TEM-1 beta-lactamases in klebsiella pneumonia
isolates through PCR method. In this descriptive-analytic study,120klebsiella pneumonia isolates collected from
patients with Lung infection and UTI were subjected to bacteriological tests. The samples were cultured and
identified according to standard methods. Then, frequency of the strains producing extended spectrum betalactamases
was determined with Disk diffusion method. Byusing of kits, DNA was extracted and examined for the
existence of TEM-1 gene by PCR. out of the 120 klebsiella pneumonia isolates, 13 (10.83 %) isolates were ESBL
positive, 5.3 % of which were positive for TEM-1 B-lactamase resistance gene. Considering the increasing rate of
the ESBLs producing strains, using the appropriate treatment protocol based on the antibiogram pattern of the
strains is highly recommended.

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