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The Effect of Glycine Treatment on Histomorphometric Changes in the Diabetic Rat Retina

Authors: Soghra Gholami and Mohammad Reza Rostamzad

Int J Med Res Health Sci.288-292 | pdf PDF Full Text

Diabetic retinopathy is a major cause of blindness and one of its possible mechanisms is impairment in proteins and
amino acids synthesis due to impairment in glucose synthesis. So far, different amino acids have been used to
improve diabetic retinopathy. However, no research has been done till now about the effect of glycine on
histological changes of diabetic rats retina. So, this is the main goal of the current study. 40 adult male Wistar rats
weighing approximately 200- 250 g were prepared and divided into 4 groups of 10 each, which include: the first
group (the rats were kept without receiving any material), the second group (healthy, the rats were received glycine
at concentration of 130 mM, 1 %w/v), the third group (diabetic, the rats were kept without receiving any material),
the fourth group (diabetic, the rats were received glycine at concentration of 130 mM, 1 %w/v). All rats were
anesthetized after 16 weeks The separated retina was immersed in glutaraldehyde 4% for 3-4 h. Retina tissue was
rised with buffer, post fixed in osmium tetroxide 1% and dehydrated through a graded ethanol series. The tissue was
then placed in a mixture of propylene oxide and TAAB resin and transferred in pure resin. Semithin sections was
prepared and viewed under light microscope (LM). Ultrathin sections were prepared, stained, and Viewed under
TEM. Studies and evaluation of the thickness of IPL, INL, OPL, ONL, PSL and GCL. After preparing slides from the
samples and staining with toluidine blue stain, they were examined and compared with light microscopy. The results
showed that a significant decrease in the thickness of PSL and ONL and a significant increase in the thickness of
IPL were observed in the group received glycine compared to the normal group (P≤0.05). There was a decrease in
the thickness of all layers except PSL and IPL in the diabetic group compared to the normal group. These changes
were not statistically significant. The thickness of whole retina and its layers were increased significantly in diabetic
group received glycine compared to other groups (P≤0.05). Glycine increases the thickness of retinal layers in the
retinopathic group through its anti-diabetic properties as well as its influence on improvement of protein synthesis
in retina; so, treatment with glycine is recommended to improve different layers of retina in diabetic patients with
retinopathy.
Keywords: Glycine, Histomorphomeric, Retina, Diabetes

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