A special issue on Nursing: Current Scenario and Future Development - Articles are invited.

Molecular Identification of Cholesterol Oxidase Enzyme-Producing Streptomyces Bacteria in Soil of Lorestan Province, Iran

Authors: HamedEsmaeilLashgarian, SarehJahanbakhsh and Kiana Shahzamani

Int J Med Res Health Sci.54-62 | pdf PDF Full Text

Bacterial Cholesterol oxidase (CHO) is a monomeric, bi-functional and Flavin adenine dinucleotide-linked enzyme
that catalyzes oxidation and isomerization cholesterol in the presence of molecular oxygen. Recently, powerful
pesticide properties of these enzymes have been discovered on pest and in agriculture was used as a new generation
of biological pesticides in cotton plants. This study aimed to isolate and molecular identification of Streptomyces
bacteria producing CHO from Lorestan soil to provide biological pesticide in future. A total of 100 samples were
collected from garden and agricultural land soils of Lorestan province. First, samples were cultured in cholesterolcontaining medium as an alone carbon source. Identification of grown bacteria was performed using the
morphological characteristics, biochemical and microbial tests. Then, the colorimetric and browning of culture
medium confirmed the activity of CHO. Then, the activity of cholesterol oxides was assayed under various
temperature and pH. Obtained strains were confirmed by amplification of 16s rRNA gene using PCR technique and
sequencing. A total of three producing cholesterol oxidase bacterial samples were isolated. The results showed that
obtained strains belong to Streptomyces. The activity of cholesterol oxidase enzyme was confirmed by the
colorimetric, and browning of culture medium tests. Finally, the molecular identity of the bacterial species was
detected by sequencing PCR products. The results showed that the bacterial species, Streptomyces, isolated from the
soil is capable of producing cholesterol oxidase enzyme.

International Journal of Medical Research & Health Sciences © 2012-16 [Last updated: July 2016] by AspiringDigital