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Investigation of protein in blood serum of dengue patients by SDS-PAGE technique

Authors: Iram Khan, Zaid Mehmood, Anum Waseem and Jahanara Hassan

Int J Med Res Health Sci.72-77 | pdf PDF Full Text

Dengue viruses belong to the genus flavivirus within the Flaviviridae family. It is self limited viral infection that is transmitted between people by mosquitoes. The dengue virus genome encodes three structural and seven nonstructural proteins. Dengue virus is categorized into four serotypes,so far it is the major health problem and 2.5 billion people are at danger of this disease among them about 975 million of them live in urban areas in tropical areas of Southeast Asia. The sample of dengue positive patients were collected and Serum protein of dengue and healthy individuals can be analyzed and exploited by using a technique sodium dodecyl sulphate Polyacrylamide gel electrophoresis .For this purpose serum of healthy and infected patients were taken and infection in individuals were confirmed by using ELISA IgG, IgM and NS1 antigen test. From the SDS –PAGE analysis a protein profile with molecular weight ranging from 200kda to 10kda were analyzed .One serum protein marker that has a molecular weight of about 48kda that is specifically present in dengue patients but not in the healthy person . The recent research concluded that method adopted for protein analysis is sensitive and reliable. Careful and consistent investigation is required in the future to identify these serum protein marker and functions of these protein in the dengue disease.

Keywords: Dengue patients, sodium dodecyl sulphate Polyacrylamide gel electrophoresis,, ELISA, Serum protein marker.

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