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Influence of physical exercise on interleukin-17, cortisol and melatonin levels in serum, whole blood and mitogen activated peripheral blood mononuclear cells

Authors: Zarei M., Farokhi Fard G., Salehi I., Sarihi A. and Zamani A.

Int J Med Res Health Sci.77-83 | pdf PDF Full Text

The purpose of this study was to assay the effect of two months exercise and two months silent on the levels of
interleukin-17 (IL-17), melatonin and cortisol in serum, whole blood (WB) and peripheral blood mononuclear cells
(PBMCs) cultures. Thirteen male non-athletic health volunteers participated in a two months moderate exercise
program (running %50-%65 VO2 max). The blood samples were collected in three stages, 24 hours before to start
exercise, 48 hours and two months after the last session of the training. WB and PBMCs were cultured with
mitogens phytohemagglutinin and lipopolysaccharides for 48 hours. The serum and supernatants of WB and
PBMCs were analyzed for IL-17, melatonin and cortisol by enzyme-linked immunosorbent assay. Red blood cells
(RBC) variables were also measured. IL-17 secretion by PBMCs in the post-exercise stage (51.14±5.43 pg /ml)
compared with pre-exercise (36.74±6.98 pg/ml) was i ncreased. But, the amount of melatonin produced by PBMCs
in the post- exercise (7.94±0.35 pg/ml,) and 2-mont h silent (6.05±0.27 pg/ml) stages compared with pre -exercise
(9.16±0.19 pg/ml) were decreased. Regardless of the effect of the exercise, PBMcs had more ability to produce IL-
17 than WB. As well as, level of cortisol in WB was higher than in serum and PBMCs culture. Mean corpuscular
volume, mean corpuscular hemoglobin and mean corpuscular hemoglobin concentration RBCs were also increased
in post- exercise stage. The other measured parameters were not changed during exercise and recovery. Moderate
exercise caused to higher in vitro production of IL-17 and lower production of melatonin by PBMCs.

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