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In vitro antioxidant activity and phytochemical screening of Garhwal Himalaya medicinal plants

Authors: Subhash Chandra*, Sarla Saklani, Abhay P. Mishra and Rohit K. Agrawal

Int J Med Res Health Sci.35-43 | pdf PDF Full Text

ABSTRACT
Bistorta macrophylla, B.vaccinifolia and Persicaria polystachya are used for the treatment and prevention of many
ailments including tuberculosis, inflammation, pyretic, fever, flue, lungs disorders, diarrhea, vomiting, arthritis,
gout, kidney stones or hyperacidity and hypertension. This study was aimed to evaluate the possible in vitro
antioxidant activity and phytochemical screening of B. macrophylla, B.vaccinifolia and P. polystachya. The results
of antioxidant activity study of B. macrophylla showed maximum activity in the methanolic extracts at different
concentration of 20, 40, 60, 80 and 100 μg/ml. The percent inhibition of writhing response by the extract was
36.18%, 44.72%, 59.21%. 67.08% and 83.39% respectively. In the present work a potent anti-oxidant activity of
methanolic extract of the whole plants of B. macrophylla were demonstrated, validating the ethno pharmacological
claims. These experimental findings would further establish the scientific basis of the traditional uses of the plant in
the management of different conditions as well as control of different disease.
Keywords: Antioxidant activity, Phenolic compounds and Phytochemical screening.

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