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Expression of hsa Let-7a MicroRNA of Macrophages Infected by Leishmania Major

Authors: Nooshin Hashemi, Mohammadreza Sharifi, Sepideh Tolouei, Mitra Hashemi, Cyrus Hashemi and Seyed Hossein Hejazi

Int J Med Res Health Sci. 27-32 | pdf PDF Full Text

Leishmaniasis is a vector-born disease caused by species of the genus Leishmania and is transmitted from host to
host through the bite of an infected sandfly. MicroRNAs (miRNAs) are non-coding small RNAs with 22-nucleotide
length. They are involved in some biological and cellular processes. We aimed to evaluate the expression of let-7a
in human macrophages miRNA when are infected by Leishmania major. We also evaluated the impact of
Leishmania major infection on the expression of let-7a at two different times, 24 and 48 hours, after infection. Blood
samples were collected from ten healthy volunteers with no history of leishmaniasis. Development of macrophages
from peripheral monocytes and infection with stationary phase of Leishmania major promastigotes were done
through serial cultures under 5% CO
2 environment and 37C.To measure the expression levels of let-7a real-time
PCR was performed with specific related primers using the SYBR® Green master mix Kit™. The real-time PCR
showed let-7a was expressed in cells infected with parasites after 24 and 48h post-infection. Comparison of let-7a
miRNA expression after 24 and 48 h revealed that let-7a miRNAs were down-regulated at 48 h post-infection more
than 24h after infection. The results of this study suggest that according to the main function of miRNA in repression
of mRNA translation it could be possible to manipulate host cells in order to alter miRNA levels and regulate
macrophage functions after establishment of intracellular parasites such as Leishmania.

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