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Evaluation of the Antimicrobial Effects of Various Methods to Disinfect Toothbrushes Contaminated with Streptococcus mutans

Authors: Foroogh Amirabadi and Sepideh Sasannejad

Int J Med Res Health Sci.536-540 | pdf PDF Full Text

Toothbrushes sterilization has strongly taken into consideration in order of prohibiting of transmission and reinfection.
The differences of materials, procedures and duration, made the problem more complicated. Our objective
of the present research was assessment of antibacterial effects of different procedures which are used for
disinfection of those toothbrushes which are infected to Streptococcus mutans. 144 fresh toothbrushes were
immersed in a 0.5 McFarland culture of S. mutans for 15 minutes. The toothbrushes were then classified into 8
groups ( 6 in each group) according to their disinfecting procedure including White vinegar 50%, Sodium
hypochlorite %1, Ethyl alcohol and Povidone Iodine 10%. The disinfecting procedures which used were Microwave
and Dishwasher. Bacterial cultivation was done and microbial colonies had been counted before toothbrushes were
exposed to the above disinfectants. After exposure of toothbrushes to above disinfectants for 1, 5 and 10 minutes, the
colonies were recounted. SPSS ver.19 (Kruskal–Wallis Test) was used for statistical analysis. Bacterial cultures of
samples at Chlorhexidine group (negative control) were negative and White vinegar did not make a considerable
sense. On the other hand, Sodium hypochlorite %1, Ethyl alcohol , Povidone Iodine 10% and dishwasher decreased
the numbers of S. mutans considerably (p< %5). Microwave by increasing the time of exposition, decreased the
numbers of microbes but it was not statistically significant. Povidone Iodine 10%, Sodium hypochlorite %1 and
Ethyl alcohol, could be effective in disinfection of toothbrushes against S. mutans if be used for 10 minutes.

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