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Cytotoxic effect of Prangos Pabularia extract on HELA cell line a medicinal plant

Authors: Sakinehsalehi, Saber Zahri and Seyed Mehdi Razavi

Int J Med Res Health Sci.547-552 | pdf PDF Full Text

Cancer is regarded as a complex disease which is non-curable. Traditional medicines have been applied for the
treatment of cancers in the world. Prangospabularia DC. (Apiaceae) is a perennial herb native to mountain slopes
of the central and western Asia countries. Prangos species are widely used in folk medicine. Anticancer activity of
Prangospabularia extract against HeLa line was studied using cytotoxicity effect. Many antitumor agents, have
been reported to induce apoptotic cell death. This cell death plays a critical role in killing tumor cells in canser
therapy(1) . In order to evaluate of cytotoxicity and antioxidant properties of the plant, dried powdered of roots of
P.pabularia were Soxhlet extracted successively with n-hexan, dichloromethane (DCM) and methanol. Viability
and cytotoxicity of HeLa cell line were measured by MTT assays. The antioxidant potential of the plant extracts was
evaluated by DPPH assay. Additionally characteristic of cell death were using H&E morphological staining. The
DCM extract showed a significant antioxidant effect with RC50 value of 0.08 mg/ml and our results showed that
DCM extract displayed a significant cytotoxic activity against HeLa cell line in culture with a IC50 value of 0.526
mgml-1 in 24h with MTT assay. Additionally characteristic of aponecrotic cell death confirmed using H&E
morphological staining. This study reveals biological effects and antitumor activity of Prangospabularia
DC.(Apiaceae) extract against HeLa line.

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