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A study on the incidence of preterm rupture of membranes in pregnant women caused by Mycoplasma hominis through comparison of culture and PCR techniques in the Alavi Hospital of Ardabil Province

Authors: Arezu Ardforoosh, Behbood Jafariand Roghiyeh Dargahi

Int J Med Res Health Sci.247-253 | pdf PDF Full Text

My coplasma homin is one of the smallest free-living prokaryotes that lack peptidoglycan in the cell wall. It is also a
well-known pathogenic Mycoplasmain the human reproductive system that causes abortion, bacterial vaginosis,
infertility, and a low birth weight. This research investigated the incidence of preterm rupture of membrane (PROM)
in pregnant women due to M. hominis in the Alavi Hospital of Ardabil Province using two
comparative PCR techniques. This study evaluated diagnostic tests on 100 pregnant women with ruptured
membrane and 20 healthy women with no symptoms (control) referred to the Alavi Hospital in Ardabil
Province. After sampling, the samples were transferred to the laboratory in tubes containing PBS and PPLO. Using
culture of samples from 100 pregnant women with PROM, 41 samples (41%) were positive for the presence of
M.hominis whereas PCR revealed positive results in62 samples (62%). PCR method displayed positive cases in 2
(10%) out of 20patients in the control group. PCR results were not significantly correlated with education level,
parity, and gestational age. A direct correlation was observed between the ages of pregnant women with vaginal
infections before pregnancy, history of abortion, and in women with PROM. The incidence of PROM in pregnant
women caused by infection of the bacterium M.hominis is high. PCR technique is more appropriate compared with
the culture method to detect M.hominis positive cases in pregnant women with PROM

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